Clc sequence viewer 85/7/2023 ![]() ![]() Designing vaccines with bioinformatic approaches is a very useful way because it can identify effective epitopes and suggest more efficient vaccines. These experimental methods are often time-consuming and highly expensive. Subunit vaccines are usually developed using laboratory techniques such as cloning and expression of immune-stimulating antigens in vitro and the ability to stimulate the immune system of living organisms. In addition, since killed or attenuated bacterial vaccines have various side effects, the use of subunit vaccines will be more effective than a whole bacteria cell. ![]() Currently, there are no brucella vaccines for humans, and the designed vaccines for animals show many side effects, such as infection. The disease causes clinical manifestations, including fever, weakness, malaise and weight loss, lymphadenopathy, arthralgia/arthritis, hepatosplenomegaly, and hearing loss. It is transmitted to humans by consumption of unpasteurized foods and dairy products, inhalation of infected aerosols, or occupational contact. melitensis is the main cause of human chronic brucellosis. Four species are the main causes of infection in humans including B. It is one of the five zoonotic infections caused by gram-negative aerobic non-motile facultative intracellular coccobacillus brucella spp. According to the World Health Organization (WHO), it is one of the most neglected endemic zoonoses in the world. Graphical Abstractīrucellosis also known as Malta fever is a worldwide zoonotic disease. It showed the appropriate physicochemical properties, a high-quality structure, and a high potential for expression in a prokaryotic system. The designed vaccine showed the high ability to induce immune response, especially cellular responses to human brucellosis. The immune simulation was performed to reveal the immune response profile of the vaccine after injection. For successful expression of the vaccine protein in Escherichia coli, in silico cloning, codon optimization, and mRNA stability were evaluated. The vaccine was docked with toll-like receptor4 to assess its ability to stimulate innate immune responses. Then its two and three-dimensional structure was predicted. The physicochemical and immunological properties of the vaccine were evaluated. In order to improve its immunogenicity, suitable adjuvants were also added to the structure of the vaccine. ![]() They showed high antigenic ability without the allergenic characteristic. They had significant potential to induce cellular and humoral responses. Seven epitopes from four main brucella species that infect humans were selected. This study used advanced computational techniques to develop a potent multi-epitope vaccine for human brucellosis. Vaccination appears to be a promising health strategy to prevent it. Brucellosis is one of the neglected endemic zoonoses in the world. ![]()
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